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Nucleic Acid with Guanidinium Modification Exhibits Efficient Cellular Uptake
Author(s) -
Ohmichi Tatsuo,
Kuwahara Masayasu,
Sasaki Naoko,
Hasegawa Masatoshi,
Nishikata Takahito,
Sawai Hiroaki,
Sugimoto Naoki
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200500904
Subject(s) - oligomer , hela , nucleic acid , dna , fluorescence , fluorescence microscope , deoxyuridine , chemistry , biophysics , biochemistry , microbiology and biotechnology , combinatorial chemistry , computational biology , biology , organic chemistry , cell , physics , quantum mechanics
Quick on the uptake : A 20‐mer DNA oligomer modified with 5‐[(6‐guanidiniohexylcarbamoyl)methyl]‐2′‐deoxyuridine ( Tg ) shows efficient cellular uptake (blue arrow) in HeLa cells without any other reagents. The fluorescence microscopy image reveals FAM‐labeled DNA oligomer (green) and stained nuclei (blue). FAM=6‐carboxyfluorescein.