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Expanding the Range of Substrate Acceptance of Enzymes: Combinatorial Active‐Site Saturation Test
Author(s) -
Reetz Manfred T.,
Bocola Marco,
Carballeira José Daniel,
Zha Dongxing,
Vogel Andreas
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200500767
Subject(s) - active site , substrate (aquarium) , saturated mutagenesis , saturation (graph theory) , enzyme , substrate specificity , amino acid , lipase , combinatorial chemistry , chemistry , stereochemistry , biochemistry , mutant , computer science , mathematics , biology , combinatorics , ecology , gene
CASTing for success : The traditional problem of expanding the range of substrate acceptance of enzymes can be solved by creating focused libraries of mutants resulting from randomization of pairs of properly chosen amino acids around the active site (see example with the lipase from Pseudomonas aeruginosa , amino acid pairs are shown in the same color). CAST=combinatorial active‐site saturation test.