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A Small‐Molecule FRET Probe To Monitor Phospholipase A 2 Activity in Cells and Organisms
Author(s) -
Wichmann Oliver,
Wittbrodt Jochen,
Schultz Carsten
Publication year - 2006
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200500751
Subject(s) - förster resonance energy transfer , chemistry , fluorescence , membrane , biophysics , small molecule , phospholipase , biochemistry , enzyme , biology , physics , quantum mechanics
Watching live : The FRET reporter PENN/SATE (see picture; FRET=fluorescence resonance energy transfer) is membrane permeant and enters cells and small organisms by using a prodrug approach. Once inside cells the reporter is cleaved by phospholipases, which results in a 30‐fold change in the emission ratio