Premium
13 C‐Labeled Tyrosine Residues as Local IR Probes for Monitoring Conformational Changes in Peptides and Proteins
Author(s) -
Tremmel Sandra,
Beyermann Michael,
Oschkinat Hartmut,
Bienert Michael,
Naumann Dieter,
Fabian Heinz
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200500547
Subject(s) - tyrosine , chemistry , biochemistry
Launching a probe : Introducing individual tyrosine residues labeled with 13 C at the C4 position of the phenol ring (Y11 and Y19 in the picture) shifts the resulting tyrosine IR band to lower frequencies. This opens the door to the simultaneous monitoring of local conformational changes in different regions of proteins by IR spectroscopy, as demonstrated with a chemically synthesized analogue of a WW domain.