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Correlations between Molecular Numbers and Molecular Masses in an Evanescent Field and Their Applications in Probing Molecular Interactions
Author(s) -
Gai Hongwei,
Wang Qi,
Ma Yinfa,
Lin Bingcheng
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200500461
Subject(s) - evanescent wave , molecule , fluorescence , molecular imaging , fluorescein isothiocyanate , fluorescein , chemical physics , field (mathematics) , chemistry , nanotechnology , materials science , optics , physics , biology , mathematics , organic chemistry , microbiology and biotechnology , in vivo , pure mathematics
Complex numbers : The number of molecules detected in the evanescent field during single‐molecule fluorescence imaging decreases with increasing molecular weight in free solution (see graphic; FITC=fluorescein isothiocyanate). This technique could be useful for screening undefined molecular interactions at extremely low concentrations without separation.