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A Synthetic Block Copolymer Regulates S1 Nuclease Fragmentation of Supercoiled Plasmid DNA
Author(s) -
Osada Kensuke,
Yamasaki Yuichi,
Katayose Satoshi,
Kataoka Kazunori
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200500201
Subject(s) - nuclease , dna , restriction enzyme , dna supercoil , plasmid , copolymer , cationic polymerization , macromolecule , chemistry , cleavage (geology) , biophysics , biochemistry , biology , polymer , polymer chemistry , dna replication , paleontology , organic chemistry , fracture (geology)
Split into seven sizes : Highly ordered cleavage of supercoiled plasmid DNA by S1 nuclease was accomplished through DNA complexation with water‐soluble cationic block copolymers (see schematic representation). The results provide insights into the mechanisms of endogenous protein‐induced modification of DNA and into the design of artificial restriction enzymes through the supramolecular assembly of synthetic macromolecules.