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Single‐Molecule Experiments in Synthetic Biology: An Approach to the Affinity Ranking of DNA‐Binding Peptides
Author(s) -
Eckel Rainer,
Wilking Sven David,
Becker Anke,
Sewald Norbert,
Ros Robert,
Anselmetti Dario
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200500152
Subject(s) - dna , peptide , computational biology , activator (genetics) , transcription (linguistics) , dna sequencing , dna binding site , chemistry , biology , gene , microbiology and biotechnology , genetics , biochemistry , promoter , gene expression , philosophy , linguistics
Peptide–DNA recognition : The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single‐molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single‐point mutations on specific binding.