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Enhanced Fidelity in Mismatch Extension by DNA Polymerase through Directed Combinatorial Enzyme Design
Author(s) -
Summerer Daniel,
Rudinger Nicolas Z.,
Detmer Ilka,
Marx Andreas
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200500047
Subject(s) - dna polymerase , polymerase , dna , computational biology , chemistry , primer extension , genotyping , fidelity , combinatorial chemistry , computer science , biology , biochemistry , base sequence , gene , genotype , telecommunications
How is a selective enzyme made even more selective? With a combinatorial approach to redesign an enzyme motif (see figure; red=O, blue=N), the mismatch extension selectivity of DNA polymerases was increased. The results impart new and deeper insight into the mechanisms of DNA polymerases and provide new tools for applications such as efficient genotyping.