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Mutasynthesis of Rapamycin Analogues through the Manipulation of a Gene Governing Starter Unit Biosynthesis
Author(s) -
Gregory Matthew A.,
Petkovic Hrvoje,
Lill Rachel E.,
Moss Steven J.,
Wilkinson Barrie,
Gaisser Sabine,
Leadlay Peter F.,
Sheridan Rose M.
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200462784
Subject(s) - starter , streptomyces hygroscopicus , gene cluster , polyketide , gene , mutant , biosynthesis , product (mathematics) , computer science , biology , chemistry , computational biology , streptomyces , biochemistry , genetics , food science , mathematics , bacteria , geometry
The surprising discovery has been made that the gene product RapK is required for production of the starter unit in the rapamycin polyketide cluster. It is shown that a deletion mutant ( Streptomyces hygroscopicus MG2–10) provides a clean background for the mutasynthesis of pre‐rapamycin (the precursor to rapamycin, see structure) when fed with novel starter units.