Kinetics of Photoinduced RNA Refolding by Real‐Time NMR Spectroscopy | Zendy

Wenter Philipp | Zendy; Fürtig Boris | Zendy; Hainard Alexandre | Zendy; Schwalbe Harald | Zendy; Pitsch Stefan | Zendy

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Kinetics of Photoinduced RNA Refolding by Real‐Time NMR Spectroscopy

Author(s) -

Wenter Philipp,

Fürtig Boris,

Hainard Alexandre,

Schwalbe Harald,

Pitsch Stefan

Publication year - 2005

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200462724

Subject(s) - guanosine , chemistry , base pair , rna , nuclear magnetic resonance spectroscopy , kinetics , sequence (biology) , bistability , pulse sequence , crystallography , stereochemistry , nuclear magnetic resonance , dna , physics , biochemistry , quantum mechanics , gene

By introducing a photolabile group in the Watson–Crick base‐pairing site of a guanosine residue, a bistable 20‐base RNA sequence was forced into a less stable conformation. A single laser pulse released the native sequence, and the subsequent refolding equilibration was monitored with time‐resolved NMR spectroscopy (see spectra). This permitted a quantitative description of the refolding process.

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