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Kinetics of Photoinduced RNA Refolding by Real‐Time NMR Spectroscopy
Author(s) -
Wenter Philipp,
Fürtig Boris,
Hainard Alexandre,
Schwalbe Harald,
Pitsch Stefan
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200462724
Subject(s) - guanosine , chemistry , base pair , rna , nuclear magnetic resonance spectroscopy , kinetics , sequence (biology) , bistability , pulse sequence , crystallography , stereochemistry , nuclear magnetic resonance , dna , physics , biochemistry , quantum mechanics , gene
By introducing a photolabile group in the Watson–Crick base‐pairing site of a guanosine residue, a bistable 20‐base RNA sequence was forced into a less stable conformation. A single laser pulse released the native sequence, and the subsequent refolding equilibration was monitored with time‐resolved NMR spectroscopy (see spectra). This permitted a quantitative description of the refolding process.