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Recognizing a Single Base in an Individual DNA Strand: A Step Toward DNA Sequencing in Nanopores
Author(s) -
Ashkenasy Nurit,
SánchezQuesada Jorge,
Bayley Hagan,
Ghadiri M. Reza
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200462114
Subject(s) - library science , chemistry , computer science
The capture of single strands of DNA inside the α‐hemolysin (α‐HL) transmembrane pore protein upon application of a positive potential leads to single α‐HL⋅DNA pseudorotaxane species (see picture). By monitoring the characteristic decreases in the ion conductance of α‐HL, a single adenine nucleotide can be identified at a specific location on a strand of DNA.