Premium
High‐Throughput Protein Glycomics: Combined Use of Chemoselective Glycoblotting and MALDI‐TOF/TOF Mass Spectrometry
Author(s) -
Nishimura ShinIchiro,
Niikura Kenichi,
Kurogochi Masaki,
Matsushita Takahiko,
Fumoto Masataka,
Hinou Hiroshi,
Kamitani Ryousuke,
Nakagawa Hiroaki,
Deguchi Kisaburo,
Miura Nobuaki,
Monde Kenji,
Kondo Hirosato
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200461685
Subject(s) - glycomics , mass spectrometry , chemistry , matrix assisted laser desorption/ionization , throughput , chromatography , glycoprotein , computer science , glycan , biochemistry , organic chemistry , adsorption , telecommunications , desorption , wireless
Common oligosaccharides and aldehyde‐attached glycoproteins derived from enzymatic modifications can be captured by glycoblotting based on oxylamino‐containing polymers. The combination of this glycoblotting and MALDI‐TOF/TOF mass spectrometry enabled a high‐throughput analytical method to be developed.