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Direct Characterization of Enzyme–Substrate Complexes by Using Electrosonic Spray Ionization Mass Spectrometry
Author(s) -
Wiseman Justin M.,
Takáts Zoltán,
Gologan Bogdan,
Davisson V. J.,
Cooks R. Graham
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200461672
Subject(s) - electrospray ionization , chemistry , extractive electrospray ionization , ionization , mass spectrometry , biomolecule , direct electron ionization liquid chromatography–mass spectrometry interface , ternary operation , non covalent interactions , imidazole , chromatography , analytical chemistry (journal) , sample preparation in mass spectrometry , molecule , chemical ionization , hydrogen bond , stereochemistry , organic chemistry , ion , biochemistry , computer science , programming language
It's a phase it's going through : Electrosonic spray ionization shows better desolvation efficiency than electrospray ionization and usually gives single‐charge states when used to ionize noncovalent complexes of large biomolecules. The binary and ternary complexes of imidazole‐3‐glycerol phosphate synthase (IGPS) with its inhibitors appear to retain their native solution‐phase conformations upon transfer into the gas phase (see picture).