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Activity‐Based High‐Throughput Screening of Enzymes by Using a DNA Microarray
Author(s) -
Hu Yi,
Chen Grace Y. J.,
Yao Shao Q.
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200461612
Subject(s) - complementary dna , dna , computational biology , microarray , reverse transcriptase , microbiology and biotechnology , transcription (linguistics) , polymerase chain reaction , chemistry , dna microarray , enzyme , biology , gene , gene expression , biochemistry , linguistics , philosophy
The expression display method is developed, which combines two techniques, ribosome display and DNA microarray, to express a mixture of proteins from their corresponding cDNA. Upon functional selection using an activity‐based probe, multiple target enzymes are simultaneously isolated and subsequently identified by hybridization to a DNA microarray (see scheme; RT=reverse transcription, PCR=polymerase chain reaction).