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Highly Efficient Biosynthesis of the Oligosaccharide Moiety of the GD3 Ganglioside by Using Metabolically Engineered Escherichia coli
Author(s) -
Antoine Tatiana,
Heyraud Alain,
Bosso Claude,
Samain Eric
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200461507
Subject(s) - campylobacter jejuni , escherichia coli , sialyltransferase , biosynthesis , biochemistry , ganglioside , sialic acid , chemistry , oligosaccharide , glycosyltransferase , gene , biology , bacteria , genetics
Express order for oligosaccharides : A microbiological process for the synthesis of the carbohydrate portion of gangliosides GD3 and GT3 is described (see structure). Lactose and sialic acid are used as exogenous precursors by a metabolically engineered Escherichia coli strain that overexpresses the bifunctional sialyltransferase cstII gene from Campylobacter jejuni.