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A Direct Glimpse of Cross‐Hybridization: Background‐Passified Microarrays That Allow Mass‐Spectrometric Detection of Captured Oligonucleotides
Author(s) -
Plutowski Ulrich,
Richert Clemens
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200461212
Subject(s) - oligonucleotide , monolayer , mass spectrometry , chemistry , dna , dna microarray , analytical chemistry (journal) , nanotechnology , computational biology , chromatography , materials science , gene , biochemistry , biology , gene expression
Oligonucleotides immobilized in SAMs (self‐assembled monolayers) with a passifying surface layer, prepared from thermally smoothed gold surfaces and disulfides, allow the direct detection of bound strands by MALDI‐TOF mass spectrometry. The binding of perfectly matched (PM) and mismatched (MM) DNA targets is observed (see picture). The extent to which cross‐hybridization occurs depends on the type and position of the mismatch.