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DNA Detection through Signal Amplification by Using NADH:Flavin Oxidoreductase and Oligonucleotide–Flavin Conjugates as Cofactors
Author(s) -
Simon Philippe,
Dueymes Cécile,
Fontecave Marc,
Décout JeanLuc
Publication year - 2005
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200461145
Subject(s) - cofactor , flavin group , oligonucleotide , conjugate , substrate (aquarium) , enzyme , dna , oligomer restriction , oxidoreductase , chemistry , biochemistry , combinatorial chemistry , biology , mathematical analysis , ecology , mathematics
Cofactors on the case : DNA detection by using an enzyme and its cofactor attached to an oligonucleotide is described. If the enzyme is functional only when the cofactor–oligonucleotide conjugate is not hybridized, substrate conversion monitored by fluorescence spectroscopy can be used as a reaction probe for hybridization (see scheme). Furthermore, as the enzyme turns over many copies of the substrate, the signal is greatly amplified.