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Photocontrol of Smad2, a Multiphosphorylated Cell‐Signaling Protein, through Caging of Activating Phosphoserines
Author(s) -
Hahn Michael E.,
Muir Tom W.
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200461141
Subject(s) - phosphoserine , cell , microbiology and biotechnology , chemistry , biochemistry , biology , computational biology , phosphorylation , serine
Expressed‐protein ligation has been used for the semisynthetic preparation of a Smad2 protein with caged activating phosphoserine residues. Biochemical and cell biological experiments show that the caged protein is activated upon irradiation with UV light (see scheme). This sets the stage for the detailed kinetic analysis of Smad2 function in living cells.