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Design and Synthesis of Photochemically Controllable Caspase‐3
Author(s) -
Endo Masayuki,
Nakayama Koji,
Kaida Yuka,
Majima Tetsuro
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200460889
Subject(s) - cleavage (geology) , caspase 3 , chemistry , residue (chemistry) , caspase 8 , peptide , caspase , computer science , combinatorial chemistry , biochemistry , engineering , apoptosis , programmed cell death , fracture (geology) , geotechnical engineering
The selective cleavage achieved by activated caspase‐8 has been mimicked by using a photofunctionalized caspase‐3 having 2‐nitrophenylglycine (Npg) at a specific position of the peptide chain (see picture). The study shows that the activity of caspase‐3 has been clearly expressed by photoirradiation and that autocleavage of caspase‐3 has been suppressed by the site‐selective incorporation of the Npg residue.