Premium
An Amphotericin B–Fluorescein Conjugate as a Powerful Probe for Biochemical Studies of the Membrane
Author(s) -
Zumbuehl Andreas,
Jeannerat Damien,
Martin Scott E.,
Sohrmann Marc,
Stano Pasquale,
Vigassy Tamas,
Clark Daniel D.,
Hussey Stephen L.,
Peter Mathias,
Peterson Blake R.,
Pretsch Ernö,
Walde Peter,
Carreira Erick M.
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200460489
Subject(s) - amphotericin b , conjugate , fluorescein , linker , in vivo , mechanism of action , chemistry , liposome , ex vivo , pharmacology , biochemistry , biology , in vitro , fluorescence , computer science , antifungal , microbiology and biotechnology , mathematical analysis , physics , mathematics , quantum mechanics , operating system
Not only skin deep : A fluorescein–amphotericin B conjugate with a new piperazine linker (see structure) was prepared and used as a probe of the biomembrane and the mechanism of action of amphotericin B both in vivo and in liposomal experiments. The amphotericin B analogue localizes at the membrane of yeast cells but is internalized by mammalian cells.