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Individual Molecules of Dye‐Labeled DNA Act as a Reversible Two‐Color Switch upon Application of an Electric Field
Author(s) -
White Samuel S.,
Ying Liming,
Balasubramanian Shankar,
Klenerman David
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200460323
Subject(s) - förster resonance energy transfer , electric field , fluorescence , molecule , acceptor , field (mathematics) , quantum yield , photochemistry , dna , energy transfer , materials science , chemistry , chemical physics , optoelectronics , nanotechnology , optics , physics , condensed matter physics , organic chemistry , biochemistry , mathematics , quantum mechanics , pure mathematics
Reversible switching of the fluorescence of an individual dye‐labeled DNA molecule is possible by utilizing an electric field applied in the tip of a nanopipette (see picture; FRET=fluorescence resonance energy transfer). The electric field appears to alter the conformation of the acceptor dye only, resulting in a significant change in its quantum yield.