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A Protein‐Driven DNA Device That Measures the Excess Binding Energy of Proteins That Distort DNA
Author(s) -
Shen Wanqiu,
Bruist Michael F.,
Goodman Steven D.,
Seeman Nadrian C.
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200460302
Subject(s) - förster resonance energy transfer , dna , fluorescence , binding energy , dna binding site , dna binding protein , binding site , crystallography , biophysics , chemistry , biology , physics , transcription factor , atomic physics , gene , biochemistry , optics , promoter , gene expression
A DNA nanomechanical device was constructed by linking two triple‐helical DNA motifs with a shaft of conventional DNA bearing the binding site for the integration host factor (IHF, shown as green ellipse in the picture). Binding of IHF to this site causes marked shaft bending, which can be monitored by fluorescence resonance energy transfer between donor (D) and acceptor (A) dyes.