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Efficient Whole‐Cell Biotransformation in a Biphasic Ionic Liquid/Water System
Author(s) -
Pfruender Holger,
Amidjojo Maya,
Kragl Udo,
WeusterBotz Dirk
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200460241
Subject(s) - biotransformation , ionic liquid , biocatalysis , chemistry , cofactor , substrate (aquarium) , biocompatible material , ethanol , combinatorial chemistry , product inhibition , chromatography , chemical engineering , biochemistry , catalysis , enzyme , non competitive inhibition , biomedical engineering , biology , medicine , engineering , ecology
Biocompatible ionic liquids act as a substrate reservoir and an in situ extracting agent in biotransformations with whole cells (see scheme). In the reduction of 4‐chloroacetophenone to ( R )‐1‐(4‐chlorophenyl)ethanol, high product concentration (82 g L −1 ) and purity (99.6 % ee ) were achieved without the addition of cofactors and without damaging the biocatalyst.