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Post‐Translational Modifications on Proteins: Facile and Efficient Procedure for the Identification of O‐Glycosylation Sites by MALDI‐LIFT‐TOF/TOF Mass Spectrometry
Author(s) -
Kurogochi Masaki,
Matsushita Takahiko,
Nishimura ShinIchiro
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200460020
Subject(s) - glycopeptide , glycosylation , chemistry , tandem mass spectrometry , mass spectrometry , matrix assisted laser desorption/ionization , lift (data mining) , fragmentation (computing) , chromatography , biochemistry , computer science , organic chemistry , data mining , adsorption , desorption , operating system , antibiotics
We have LIFT‐TOF ! Transglycosylation of glycopeptides by β‐galactosidase in the presence of lactose combined with tandem mass spectrometry using a 2,5‐dihydroxybenzoic acid matrix enables the O‐glycosylation sites to be identified (see scheme). The highly sensitive and ideal fragmentation patterns generated from glycopeptide precursor ion peaks can be readily used for precise and reliable structure analyses.