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An SECM Detection Scheme with Improved Sensitivity and Lateral Resolution: Detection of Galactosidase Activity with Signal Amplification by Glucose Dehydrogenase
Author(s) -
Zhao Chuan,
Wittstock Gunther
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200454261
Subject(s) - scanning electrochemical microscopy , ultramicroelectrode , chemistry , biosensor , resolution (logic) , signal (programming language) , biophysics , nanotechnology , electrochemistry , analytical chemistry (journal) , materials science , electrode , biochemistry , chromatography , cyclic voltammetry , programming language , biology , artificial intelligence , computer science
An enzymatic amplification cycle is used in a novel detection scheme for scanning electrochemical microscopy (SECM). An immobilized enzyme system comprising galactosidase and PQQ‐dependent glucose dehydrogenase was imaged by SECM with high sensitivity and high lateral resolution. PQQ=pyrroloquinolinequinone.