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Label‐Free Electrochemical Recognition of DNA Hybridization by Means of Modulation of the Feedback Current in SECM
Author(s) -
Turcu Florin,
Schulte Albert,
Hartwich Gerhard,
Schuhmann Wolfgang
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200454228
Subject(s) - scanning electrochemical microscopy , oligonucleotide , electrochemistry , chemistry , modulation (music) , amperometry , dna , dna–dna hybridization , biophysics , electrode , nanotechnology , materials science , biochemistry , biology , physics , acoustics
Stranded on the surface : The modulation of the amperometric feedback current in scanning electrochemical microscopy (SECM) through coulomb interactions between the phosphate groups of immobilized oligonucleotides and a freely diffusing, negatively charged redox mediator is used for imaging DNA spots (see picture) and establishing a simple and efficient label‐free electrochemical detection of complementary hybridization on DNA microarrays.