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Monitoring Molecular Recognition of the Ribosomal Decoding Site
Author(s) -
Shandrick Sarah,
Zhao Qiang,
Han Qing,
Ayida Benjamin K.,
Takahashi Masayuki,
Winters Geoffrey C.,
Simonsen Klaus B.,
Vourloumis Dionisios,
Hermann Thomas
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200454217
Subject(s) - rna , ribosomal rna , chemistry , computational biology , conformational change , ligand (biochemistry) , domain (mathematical analysis) , computer science , stereochemistry , biophysics , biochemistry , biology , gene , mathematics , mathematical analysis , receptor
Adenine flips out : A combination of X‐ray crystallography, 2‐aminopurine fluorescence labeling, and the use of aminoglycosides as ligands is exploited to demonstrate conformational transitions in the RNA domain that ensures accurate protein synthesis (see picture). The triggering of a conformational change of an adenine unit in the RNA by ligand binding can be used as the basis of a screening method to discover antibiotics.