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Use of Dynamic Combinatorial Chemistry for the Identification of Covalently Appended Residues that Stabilize Oligonucleotide Complexes
Author(s) -
Bugaut Anthony,
Toulmé JeanJacques,
Rayner Bernard
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200454041
Subject(s) - schematic , oligonucleotide , duplex (building) , covalent bond , dna , combinatorial chemistry , chemistry , rna , representation (politics) , computer science , computational biology , biochemistry , biology , organic chemistry , engineering , gene , electronic engineering , politics , law , political science
Amplification with kissing : Rapid identification of covalently appended residues that stabilize oligonucleotide complexes has been achieved by using a dynamic combinatorial approach that leads to amplification of the best ligand (see schematic representation). Both a DNA duplex and an RNA loop–loop (kissing) complex were investigated. Melting‐temperature experiments confirmed that the amplified species stabilize the complexes.