Premium
Alternative Translations of a Single RNA Message: An Identity Switch of (2 S ,3 R )‐4,4,4‐Trifluorovaline between Valine and Isoleucine Codons
Author(s) -
Wang Pin,
Fichera Alfio,
Kumar Krishna,
Tirrell David A.
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200454036
Subject(s) - aminoacylation , valine , isoleucine , transfer rna , translation (biology) , rna , biology , genetics , amino acid , computational biology , leucine , messenger rna , gene
Changed in translation : Bacterial expression hosts can be engineered so that a single RNA message can be read in different ways depending on the relative rates of competing aminoacylation reactions. The (2 S ,3 R )‐4,4,4‐trifluorovaline can be assigned either to isoleucine or to valine codons according to whether the bacterial host overexpresses the isoleucyl‐ or the valyl‐tRNA synthetase (IleRS and ValRS, respectively; see scheme).