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Simultaneous Identification of Multiple Protein Targets by Using Complementary‐DNA Phage Display and a Natural‐Product‐Mimetic Probe
Author(s) -
McKenzie Kathleen M.,
Videlock Elizabeth J.,
Splittgerber Ute,
Austin David J.
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200454004
Subject(s) - phage display , cloning (programming) , dna , computational biology , selection (genetic algorithm) , identification (biology) , phagemid , microbiology and biotechnology , computer science , chemistry , biology , genetics , gene , bacteriophage , artificial intelligence , escherichia coli , programming language , antibody , botany
Putting on a good display : A novel selection protocol was devised for the simultaneous display cloning of three homologues of the FK506‐binding protein (FKBP12, FKBP12.6, and FKBP13) by using AP1497 (see structure), a mimetic of FK506, and a T7 complementary‐DNA phage‐display library. A quantitative on‐phage binding assay was also performed to evaluate the affinity of the isolated proteins.