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Generation and Enzymatic Amplification of High‐Density Functionalized DNA Double Strands
Author(s) -
Jäger Stefan,
Famulok Michael
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200453926
Subject(s) - heteroduplex , primer (cosmetics) , dna , dna polymerase , base pair , nucleobase , polymerase , chemistry , extension (predicate logic) , computational biology , combinatorial chemistry , stereochemistry , microbiology and biotechnology , biology , computer science , biochemistry , programming language , organic chemistry
Up to eight modifications could be incorporated into a DNA heteroduplex (see picture) by introducing different sets of 2′‐deoxynucleotide derivatives through DNA polymerase mediated primer extension. All four natural nucleobases in each strand were substituted with different base‐modified analogues. PCR conditions are described that allow the direct amplification of fully functionalized DNA double strands.