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Magnetic Sensors for Protease Assays
Author(s) -
Zhao Ming,
Josephson Lee,
Tang Yi,
Weissleder Ralph
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200390352
Subject(s) - biotinylation , nanosensor , protease , avidin , superparamagnetism , cleavage (geology) , chemistry , biotin , conjugated system , nanotechnology , enzyme , biophysics , biochemistry , materials science , biology , magnetic field , physics , polymer , organic chemistry , magnetization , quantum mechanics , fracture (geology) , composite material
Superparamagnetic nanosensors conjugated to avidin (CLIO‐A) can be used to measure sensitively and quantitively the proteolytic cleavage of bi‐biotinylated peptide substrates (rods in the scheme) by changes in magnetic resonance properties. This approach may be generalized to determine the activity of a wide range of hydrolytic enzymes.