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Towards Multistep Nanostructure Synthesis: Programmed Enzymatic Self‐Assembly of DNA/Gold Systems
Author(s) -
Kanaras Antonios G.,
Wang Zhenxin,
Bates Andrew D.,
Cosstick Richard,
Brust Mathias
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200390075
Subject(s) - dna , dna ligase , restriction enzyme , nanostructure , nanotechnology , colloidal gold , enzyme , chemistry , materials science , biophysics , biochemistry , biology , nanoparticle
Enzyme‐dependent assembly of DNA/gold nanostructures is achieved using DNA‐capped gold colloids and DNA processing enzymes. Particle‐bound DNA double strands are cleaved at designated sites by a restriction endonuclease (see picture). In a subsequent step the particles are linked to each other through these activated sites by a DNA ligase. This new strategy enables the multistep synthesis of nanostructures.