Premium
Enzyme Microarrays: On‐Chip Determination of Inhibition Constants Based on Affinity‐Label Detection of Enzymatic Activity
Author(s) -
Eppinger Jörg,
Funeriu Daniel P.,
Miyake Masato,
Denizot Lucile,
Miyake Jun
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200353623
Subject(s) - dna microarray , enzyme , microarray , chemistry , chip , enzyme inhibition , computational biology , high throughput screening , biochemistry , microbiology and biotechnology , biology , computer science , gene expression , gene , telecommunications
High‐throughput screening of libraries of potential inhibitors is possible by using an enzyme microarray method that combines low sample consumption with accuracy and speed. Information about enzyme inhibition can be obtained “on‐chip” by the addition of a fluorescently tagged affinity label to a microarray of functional enzymes (see picture).