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Simultaneous Assignment and Structure Determination of Protein Backbones by Using NMR Dipolar Couplings
Author(s) -
Jung YoungSang,
Sharma Mukesh,
Zweckstetter Markus
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200353588
Subject(s) - nuclear magnetic resonance spectroscopy , chemistry , spectroscopy , dipole , crystallography , computer science , physics , stereochemistry , organic chemistry , quantum mechanics
Running on automatic : Folds for proteins up to around 125 residues can be achieved rapidly and without manual intervention by integration of bioinformatics tools with NMR spectroscopy in dilute liquid‐crystalline phases (see picture). Data intensive and time‐consuming assignment of backbone resonances prior to structure determination is not required any more. This greatly accelerates structure determination of proteins by NMR spectroscopy.