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A One‐Pot Total Synthesis of Crambin
Author(s) -
Bang Duhee,
Kent Stephen B. H.
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200353540
Subject(s) - yield (engineering) , polypeptide chain , chain (unit) , chemistry , computer science , computational biology , biochemistry , biology , physics , amino acid , astronomy , thermodynamics
Making up for lost time : The one‐pot synthesis of crambin (see structure) with only a single final purification step gave the target protein of exceptional purity in only two days with an overall yield of ≈40 %. Three unprotected peptide segments were linked by native chemical ligation, and the polypeptide chain assumed its 3D structure without intermediate purification steps.