Premium
A One‐Pot Total Synthesis of Crambin
Author(s) -
Bang Duhee,
Kent Stephen B. H.
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200353540
Subject(s) - yield (engineering) , polypeptide chain , chain (unit) , chemistry , computer science , computational biology , biochemistry , biology , physics , amino acid , astronomy , thermodynamics
Making up for lost time : The one‐pot synthesis of crambin (see structure) with only a single final purification step gave the target protein of exceptional purity in only two days with an overall yield of ≈40 %. Three unprotected peptide segments were linked by native chemical ligation, and the polypeptide chain assumed its 3D structure without intermediate purification steps.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom