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Small‐Interfering‐RNA Expression in Cells Based on an Efficiently Constructed Dumbbell‐Shaped DNA
Author(s) -
Taki Masumi,
Kato Yoshio,
Miyagishi Makoto,
Takagi Yasuomi,
Taira Kazunari
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200353445
Subject(s) - dumbbell , rna , dna , rna interference , small interfering rna , computational biology , vector (molecular biology) , gene , coding (social sciences) , physics , computer science , biology , microbiology and biotechnology , genetics , mathematics , recombinant dna , physiology , statistics
A dumbbell‐shaped DNA , expected to be useful for gene therapy, was synthesized by using a novel method. The dumbbell‐shaped vector (see picture) contains a small interfering RNA (siRNA) coding sequence, and the vector was used to investigate the RNA interference (RNAi) effect inside cells.