A Genetically Encoded Fluorescent Reporter of Histone Phosphorylation in Living Cells | Zendy

Lin ChiWang | Zendy; Ting Alice Y. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

A Genetically Encoded Fluorescent Reporter of Histone Phosphorylation in Living Cells

Author(s) -

Lin ChiWang,

Ting Alice Y.

Publication year - 2004

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200353375

Subject(s) - förster resonance energy transfer , phosphorylation , histone , histone h3 , domain (mathematical analysis) , phosphoserine , fluorescence , serine , chemistry , energy transfer , microbiology and biotechnology , computational biology , biology , gene , biochemistry , physics , mathematical analysis , mathematics , quantum mechanics , molecular physics

An increase in FRET indicates phosphorylation of histone H3 at serine 28. The protein‐based reporter (see picture) responds to phosphorylation through intramolecular complexation between a substrate domain derived from histone H3 and a linked phosphoserine‐recognition domain. The reporter is also effective inside living mammalian cells. FRET=fluorescence resonance energy transfer.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research