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Synthesis and Application of Fluorescence‐Labeled Ras‐Proteins for Live‐Cell Imaging
Author(s) -
Reents Reinhard,
Wagner Melanie,
Kuhlmann Jürgen,
Waldmann Herbert
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200353265
Subject(s) - fluorophore , fluorescence , confocal microscopy , intracellular , fluorescence microscope , live cell imaging , confocal , fluorescence lifetime imaging microscopy , cell , microscopy , microbiology and biotechnology , biophysics , chemistry , biology , biochemistry , optics , pathology , medicine , physics
Seeing is believing! Semisynthetic Ras‐proteins with a fluorophore introduced at an appropriate site have been developed as probes for live‐cell imaging experiments. They are processed by the cellular machinery, and their intracellular location can then be determined by confocal laser fluorescence microscopy.