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Fluorescent Imaging of Citrate and Other Intermediates in the Citric Acid Cycle
Author(s) -
Lin Zhihong,
Wu Meng,
Schäferling Michael,
Wolfbeis Otto S.
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200353169
Subject(s) - citric acid , fluorescence , citric acid cycle , chemistry , catalytic cycle , photochemistry , biochemistry , enzyme , biophysics , biology , optics , physics
Getting the picture: Citrate and other intermediates of the citric acid cycle can be imaged by time‐resolved fluorescence with a Eu 3+ tetracycline complex as a fluorescent probe (see picture). The time resolution enables discrimination between intermediates, and enzymatic conversions are not needed.