Premium
Site‐Specific Introduction of Sialic Acid into Insulin
Author(s) -
Sato Masaaki,
Sadamoto Reiko,
Niikura Kenichi,
Monde Kenji,
Kondo Hirosato,
Nishimura ShinIchiro
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200353058
Subject(s) - sialic acid , glycosylation , insulin , mutant , amino acid residue , chemistry , point mutation , biochemistry , endocrinology , biology , peptide sequence , gene
More gain, less pain : A new long‐acting insulin (see structure) was created by the enzymatic introduction of sialyllactose into mutant insulins. The glycosylation site was introduced into the insulin by point mutation without loss of biological activity. The experimental results with mice clearly demonstrated that the introduction of a sialic acid residue is crucial in prolonging glucose‐lowering activity in the blood.