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Methotrexate Conjugates: A Molecular In Vivo Protein Tag
Author(s) -
Miller Lawrence W.,
Sable Julia,
Goelet Philip,
Sheetz Michael P.,
Cornish Virginia W.
Publication year - 2004
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200352852
Subject(s) - in vivo , conjugate , methotrexate , chemistry , computational biology , biochemistry , computer science , biology , genetics , immunology , mathematics , mathematical analysis
The labeling of proteins in living cells with fluorescent substituents is critical to the study of cell biology. A ligand–receptor approach based on the interaction between dihydrofolate reductase (DHFR) and methotrexate (Mtx) serves as a general method for labeling proteins with fluorescent or otherwise functional small molecules in vivo (see figure).