Premium
Why Does TNA Cross‐Pair More Strongly with RNA Than with DNA? An Answer From X‐ray Analysis
Author(s) -
Pallan Pradeep S.,
Wilds Christopher J.,
Wawrzak Zdzislaw,
Krishnamurthy Ramanarayanan,
Eschenmoser Albert,
Egli Martin
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200352553
Subject(s) - rna , dna , nucleic acid , duplex (building) , nucleic acid structure , chemistry , nucleic acid analogue , twist , stereochemistry , microbiology and biotechnology , biology , biochemistry , nucleic acid thermodynamics , base sequence , gene , mathematics , geometry
The TNA twist : L ‐α‐threofuranosyl (3′→2′) nucleic acid (TNA) residues adopt a C4′‐ exo pucker when incorporated into an A‐ (left) or a B‐form DNA duplex (right). The resulting intranucleotide P⋅⋅⋅P distance in TNA is very similar to that in RNA (represented by a C3′‐ endo puckered adenosine residue; green). The structural data explain earlier observations that TNA hydridizes more stably with RNA than with DNA and that RNA constitutes the better template for ligating TNA fragments.