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A Proton‐Fuelled DNA Nanomachine
Author(s) -
Liu Dongsheng,
Balasubramanian Shankar
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200352402
Subject(s) - oligonucleotide , dna , moiety , chemistry , random coil , rhodamine , duplex (building) , fluorescence , computer science , biophysics , computational biology , stereochemistry , physics , biochemistry , biology , circular dichroism , quantum mechanics
Making DNA blink : A molecular machine based on the DNA i ‐motif structure is driven by changing pH (see picture). At pH 5, oligonucleotide X* folds into the i ‐motif (closed state), and complementary strand Y adopts a random‐coil structure. At pH 8, X* unfolds and forms an extended duplex with Y. This reversible process can be monitored by means of a rhodamine green label (green circle), whose fluorescence is quenched by a dabcyl moiety (gray circle) in the closed but not in the open state.