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Synthesis and Characterization of Oligonucleotides Containing the C4′‐Oxidized Abasic Site Produced by Bleomycin and Other DNA Damaging Agents
Author(s) -
Kim Jaeseung,
Gil Jun Mo,
Greenberg Marc M.
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200352102
Subject(s) - ap site , oligonucleotide , dna , bleomycin , chemistry , biochemistry , dna damage , combinatorial chemistry , computational biology , stereochemistry , biology , genetics , chemotherapy
Selective introduction of damage : The C4′‐position of DNA serves as the site of attack for a number of DNA damaging agents, including bleomycin. The C4′‐abasic site is a major product found in damaged DNA following abstraction of the hydrogen atom from this position. The first general method for the synthesis of oligonucleotides 1 containing the C4′‐abasic site at defined positions is described (see scheme). This method should facilitate physicochemical, biochemical, and biological studies on this commonly formed DNA lesion.