Competitive Binding Assays Made Easy with a Native Marker and Mass Spectrometric Quantification | Zendy

Höfner Georg | Zendy; Wanner Klaus Theodor | Zendy

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Competitive Binding Assays Made Easy with a Native Marker and Mass Spectrometric Quantification

Author(s) -

Höfner Georg,

Wanner Klaus Theodor

Publication year - 2003

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200351806

Subject(s) - fluorophore , chemistry , competitive binding , computational biology , ligand (biochemistry) , chromatography , drug target , mass spectrometry , combinatorial chemistry , receptor , biochemistry , fluorescence , biology , physics , quantum mechanics

MS makes it possible : Competitive binding assays are a widely used tool in drug research to characterize the affinity of a ligand for a biological target. However, these assays generally require special ligands which can be detected with high sensitivity, such as radioligands or ligands coupled to a fluorophore. Now native markers may be employed in conjunction with mass spectrometric quantification. First studies demonstrate this approach in binding experiments at dopamine D 1 ‐receptors.

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