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Competitive Binding Assays Made Easy with a Native Marker and Mass Spectrometric Quantification
Author(s) -
Höfner Georg,
Wanner Klaus Theodor
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200351806
Subject(s) - fluorophore , chemistry , competitive binding , computational biology , ligand (biochemistry) , chromatography , drug target , mass spectrometry , combinatorial chemistry , receptor , biochemistry , fluorescence , biology , physics , quantum mechanics
MS makes it possible : Competitive binding assays are a widely used tool in drug research to characterize the affinity of a ligand for a biological target. However, these assays generally require special ligands which can be detected with high sensitivity, such as radioligands or ligands coupled to a fluorophore. Now native markers may be employed in conjunction with mass spectrometric quantification. First studies demonstrate this approach in binding experiments at dopamine D 1 ‐receptors.