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NMR Spectroscopic Determination of the Solution Structure of a Branched Nucleic Acid from Residual Dipolar Couplings by Using Isotopically Labeled Nucleotides
Author(s) -
van Buuren Bernd N. M.,
Schleucher Jürgen,
Wittmann Valentin,
Griesinger Christian,
Schwalbe Harald,
Wijmenga Sybren S.
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200351632
Subject(s) - nucleic acid , thymine , chemistry , nucleic acid structure , nucleotide , residual , tensor (intrinsic definition) , residual dipolar coupling , dipole , nuclear magnetic resonance spectroscopy , crystallography , stereochemistry , dna , rna , biochemistry , organic chemistry , mathematics , algorithm , pure mathematics , gene
Only a small set of magnetic‐field‐induced residual dipolar couplings is required to determine the global structure of branched nucleic acids. This is demonstrated for the example of the Holliday junction (shown schematically) after 13 C labeling in the ribose of specific thymine residues. Accurate calculation of the magnetic‐field‐induced alignment tensor for any nucleic acid conformation allows the reduction in the required number of parameters.