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A Self‐Sufficient Peroxide‐Driven Hydroxylation Biocatalyst
Author(s) -
Cirino Patrick C.,
Arnold Frances H.
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200351434
Subject(s) - hydroxylation , biocatalysis , substrate (aquarium) , chemistry , catalysis , domain (mathematical analysis) , peroxide , reductase , cytochrome p450 , heme , hemeprotein , combinatorial chemistry , computer science , organic chemistry , enzyme , reaction mechanism , biology , mathematics , ecology , mathematical analysis
Directed evolution of the heme domain of cytochrome P450 BM‐3 has resulted in a versatile, highly active peroxide‐driven hydroxylation catalyst (see picture) that requires neither NADPH nor reductase and functions in a cell‐free reaction system. This simplified, “biomimetic” catalyst is amenable to further optimization, for example, to improve stability or alter its substrate range.