Chemistry in Living Cells: Detection of Active Proteasomes by a Two‐Step Labeling Strategy | Zendy

Ovaa Huib | Zendy; van Swieten Paul F. | Zendy; Kessler Benedikt M. | Zendy; Leeuwenburgh Michiel A. | Zendy; Fiebiger Edda | Zendy; van den Nieuwendijk Adrianus M. C. H. | Zendy; Galardy Paul J. | Zendy; van der Marel Gijsbert A. | Zendy; Ploegh Hidde L. | Zendy; Overkleeft Herman S. | Zendy

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Chemistry in Living Cells: Detection of Active Proteasomes by a Two‐Step Labeling Strategy

Author(s) -

Ovaa Huib,

van Swieten Paul F.,

Kessler Benedikt M.,

Leeuwenburgh Michiel A.,

Fiebiger Edda,

van den Nieuwendijk Adrianus M. C. H.,

Galardy Paul J.,

van der Marel Gijsbert A.,

Ploegh Hidde L.,

Overkleeft Herman S.

Publication year - 2003

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200351314

Subject(s) - proteasome , moiety , in vivo , chemistry , ubiquitin , biotinylation , lysis , computational biology , biochemistry , microbiology and biotechnology , biology , stereochemistry , gene

In vivo targeting of the proteasome : Probe 1 is a cell‐permeable irreversible inhibitor that alkylates the active‐site residues of the proteasome in a Michael fashion. After cell lysis, a biotin moiety is introduced by Staudinger ligation to yield construct 2 . This strategy allows activity profiling of the catalytic activities of the proteasome in vivo.

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