Premium
Semi‐Automated Synthesis and Screening of a Large Library of Degradable Cationic Polymers for Gene Delivery
Author(s) -
Anderson Daniel G.,
Lynn David M.,
Langer Robert
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200351244
Subject(s) - cationic polymerization , gene delivery , polymer , chemistry , gene , computational biology , combinatorial chemistry , genetic enhancement , biochemistry , biology , polymer chemistry , organic chemistry
Higher delivery efficiency than conventional nonviral systems (such as poly(ethyleneimine)) have been identified in 46 polymers through cell‐based screening of a large, 2350‐member library by a high‐throughput, semi‐automated process. The transfection potential of the polymers was assessed by testing their ability to deliver luciferase expressing plasmid and green fluorescent protein plasmid (see picture) to a monkey kidney fibroblast cell line.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom