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Semi‐Automated Synthesis and Screening of a Large Library of Degradable Cationic Polymers for Gene Delivery
Author(s) -
Anderson Daniel G.,
Lynn David M.,
Langer Robert
Publication year - 2003
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200351244
Subject(s) - cationic polymerization , gene delivery , polymer , chemistry , gene , computational biology , combinatorial chemistry , genetic enhancement , biochemistry , biology , polymer chemistry , organic chemistry
Higher delivery efficiency than conventional nonviral systems (such as poly(ethyleneimine)) have been identified in 46 polymers through cell‐based screening of a large, 2350‐member library by a high‐throughput, semi‐automated process. The transfection potential of the polymers was assessed by testing their ability to deliver luciferase expressing plasmid and green fluorescent protein plasmid (see picture) to a monkey kidney fibroblast cell line.